Protein phosphorylation is a ubiquitous and key step in signaling pathways. For flow cytometry staining, the right protocol can help accurately capture these phosphorylated targets for clear-cut data.

The Cytometry and Cell Sorting Facility at Baylor College of Medicine provides training, instrumentation, technical expertise, and software for flow cytometric analysis and cell sorting. This site ...

The generation of durable CD8+ T cell memory following infection or vaccination is essential for protection against future encounters with the same or related pathogens. This process has traditionally ...

Hepatocellular carcinoma (HCC) is one of the most common and lethal cancers globally, ranking as the sixth most prevalent cancer and the third leading cause of cancer-related death 1. Despite its ...

Flow cytometry is a rapid method of cell counting and sorting, in some cases incorporating several characterization techniques such as mass spectrometry and fluorescence microscopy that can allow ...

Endothelial cells are important contributors to brain development, physiology, and disease. Although RNA sequencing has contributed to the understanding of brain endothelial cell diversity, bulk ...

Flow cytometry is a way to look closely at the features of cells or particles. A sample of blood or tissue goes into a machine called a cytometer. In less than a minute, a computer can analyze ...

Around the same time, Mack Fulwyler, an engineer working at Los Alamos National Laboratory, needed to separate particles, so he drew on existing techniques to create droplets to separate cells from a ...

Over the past decade, flow cytometry has undergone transformative advancements, notably with the adoption of spectral flow cytometry and the emergence of next-generation imaging cytometers. These ...

Human umbilical vein endothelial cells (HUVECs) were seeded at 1 × 10 4 cells per cm 2 in six-well plates. Treatment with TNF-α (2.5, 5 or 10 ng/ml) or lipopolysaccharide (0.5, 1 or 5 μg/ml) treatment ...

Extracellular vesicles released by mesenchymal stromal cells are widely studied as cell-free therapies because they deliver microRNAs that regulate inflammation, angiogenesis and tissue repair.