RNA interference (RNAi) is a method for targeted gene silencing. It is a highly valuable and widely used technique for the study of gene function and for drug discovery research. During RNAi, ...

Titrate siRNAs. Using the lowest possible concentration of siRNA may help avoid unwanted side effects, says Taylor. "Off-target effects can be dose-dependent." Before you start your study, run ...

Though an essential component of experimental design, negative controls are not implemented widely by antibody manufacturers and distributors. Neglecting this important validation step has resulted in ...

A fluorescent image of the cell taken four hours into the same experiment. At this time the quantum dot-siRNA complex is distributed throughout the cellular fluid. The dark region in the middle of the ...